Role of the proteome in providing phenotypic stability in control and ectomycorrhizal poplar plants exposed to chronic mild Pb stress.
Identifieur interne : 000143 ( Main/Exploration ); précédent : 000142; suivant : 000144Role of the proteome in providing phenotypic stability in control and ectomycorrhizal poplar plants exposed to chronic mild Pb stress.
Auteurs : Agnieszka Szuba [Pologne] ; Łukasz Marczak [Pologne] ; Rafał Kozłowski [Pologne]Source :
- Environmental pollution (Barking, Essex : 1987) [ 1873-6424 ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical : Lead, Proteome.
- Mycorrhizae, Plant Roots, Populus, Proteomics.
Abstract
Lead is a dangerous pollutant that accumulates in plant tissues and causes serious damage to plant cell macromolecules. However, plants have evolved numerous tolerance mechanisms, including ectomycorrhizae, to maintain cellular Pb2+ at the lowest possible level. When those mechanisms are successful, Pb-exposed plants should exhibit no negative phenotypic changes. However, actual molecular-level plant adjustments at Pb concentrations below the toxicity threshold are largely unknown, similar to the molecular effects of protective ectomycorrhizal root colonization. In this study, we (1) determined the molecular adjustments in plants exposed to Pb but without visible Pb stress symptoms and (2) examined ectomycorrhizal root colonization (the role of fungal biofilters) with respect to molecular-level Pb perception by plant root cells. Biochemical, microscopic, proteomic and metabolomic studies were performed to determine the molecular status of Populus × canescens microcuttings grown in agar medium enriched with 0.75 mM Pb(NO3)2. Noninoculated and inoculated with Paxillus involutus poplars were analyzed in two independent comparisons of the corresponding control and Pb treatments. After six weeks of growth, Pb caused no negative phenotypic effects. No Pb-exposed poplar showed impaired growth or decreased leaf pigmentation. Proteomic signals of intensified Pb sequestration in the plant cell wall and vacuoles, cytoskeleton modifications, H+-ATPase-14-3-3 interactions, and stabilization of protein turnover in chronically Pb-exposed plants co-occurred with high metabolomic stability. There were no differentially abundant root primary metabolites; only a few differentially abundant root secondary metabolites and no Pb-triggered ROS burst were observed. Our results strongly suggest that proteome adjustments targeting Pb sequestration and ROS scavenging, which are considerably similar but less intensive in ectomycorrhizal poplars than in control poplars due to the P. involutus biofilter (as confirmed in a mineral study), were responsible for the metabolomic and phenotypic stability of poplars exposed to chronic mild Pb stress.
DOI: 10.1016/j.envpol.2020.114585
PubMed: 32387672
Affiliations:
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Electronic address: aszuba@man.poznan.pl.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035, Kórnik</wicri:regionArea><wicri:noRegion>Kórnik</wicri:noRegion></affiliation></author><author><name sortKey="Marczak, Lukasz" sort="Marczak, Lukasz" uniqKey="Marczak L" first="Łukasz" last="Marczak">Łukasz Marczak</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznań, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznań</wicri:regionArea><wicri:noRegion>Poznań</wicri:noRegion></affiliation></author><author><name sortKey="Kozlowski, Rafal" sort="Kozlowski, Rafal" uniqKey="Kozlowski R" first="Rafał" last="Kozłowski">Rafał Kozłowski</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Geography and Environmental Sciences, Jan Kochanowski University, Universytecka 7, 24-406, Kielce, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Institute of Geography and Environmental Sciences, Jan Kochanowski University, Universytecka 7, 24-406, Kielce</wicri:regionArea><wicri:noRegion>Kielce</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32387672</idno><idno type="pmid">32387672</idno><idno type="doi">10.1016/j.envpol.2020.114585</idno><idno type="wicri:Area/Main/Corpus">000312</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000312</idno><idno type="wicri:Area/Main/Curation">000312</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000312</idno><idno type="wicri:Area/Main/Exploration">000312</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Role of the proteome in providing phenotypic stability in control and ectomycorrhizal poplar plants exposed to chronic mild Pb stress.</title><author><name sortKey="Szuba, Agnieszka" sort="Szuba, Agnieszka" uniqKey="Szuba A" first="Agnieszka" last="Szuba">Agnieszka Szuba</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035, Kórnik, Poland. 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However, plants have evolved numerous tolerance mechanisms, including ectomycorrhizae, to maintain cellular Pb<sup>2+</sup> at the lowest possible level. When those mechanisms are successful, Pb-exposed plants should exhibit no negative phenotypic changes. However, actual molecular-level plant adjustments at Pb concentrations below the toxicity threshold are largely unknown, similar to the molecular effects of protective ectomycorrhizal root colonization. In this study, we (1) determined the molecular adjustments in plants exposed to Pb but without visible Pb stress symptoms and (2) examined ectomycorrhizal root colonization (the role of fungal biofilters) with respect to molecular-level Pb perception by plant root cells. Biochemical, microscopic, proteomic and metabolomic studies were performed to determine the molecular status of Populus × canescens microcuttings grown in agar medium enriched with 0.75 mM Pb(NO<sub>3</sub>)<sub>2</sub>. Noninoculated and inoculated with Paxillus involutus poplars were analyzed in two independent comparisons of the corresponding control and Pb treatments. After six weeks of growth, Pb caused no negative phenotypic effects. No Pb-exposed poplar showed impaired growth or decreased leaf pigmentation. Proteomic signals of intensified Pb sequestration in the plant cell wall and vacuoles, cytoskeleton modifications, H<sup>+</sup>-ATPase-14-3-3 interactions, and stabilization of protein turnover in chronically Pb-exposed plants co-occurred with high metabolomic stability. There were no differentially abundant root primary metabolites; only a few differentially abundant root secondary metabolites and no Pb-triggered ROS burst were observed. Our results strongly suggest that proteome adjustments targeting Pb sequestration and ROS scavenging, which are considerably similar but less intensive in ectomycorrhizal poplars than in control poplars due to the P. involutus biofilter (as confirmed in a mineral study), were responsible for the metabolomic and phenotypic stability of poplars exposed to chronic mild Pb stress.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Automated" Owner="NLM"><PMID Version="1">32387672</PMID><DateCompleted><Year>2020</Year><Month>07</Month><Day>10</Day></DateCompleted><DateRevised><Year>2020</Year><Month>07</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-6424</ISSN><JournalIssue CitedMedium="Internet"><Volume>264</Volume><PubDate><Year>2020</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Environmental pollution (Barking, Essex : 1987)</Title><ISOAbbreviation>Environ Pollut</ISOAbbreviation></Journal><ArticleTitle>Role of the proteome in providing phenotypic stability in control and ectomycorrhizal poplar plants exposed to chronic mild Pb stress.</ArticleTitle><Pagination><MedlinePgn>114585</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0269-7491(19)37166-0</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.envpol.2020.114585</ELocationID><Abstract><AbstractText>Lead is a dangerous pollutant that accumulates in plant tissues and causes serious damage to plant cell macromolecules. However, plants have evolved numerous tolerance mechanisms, including ectomycorrhizae, to maintain cellular Pb<sup>2+</sup> at the lowest possible level. When those mechanisms are successful, Pb-exposed plants should exhibit no negative phenotypic changes. However, actual molecular-level plant adjustments at Pb concentrations below the toxicity threshold are largely unknown, similar to the molecular effects of protective ectomycorrhizal root colonization. In this study, we (1) determined the molecular adjustments in plants exposed to Pb but without visible Pb stress symptoms and (2) examined ectomycorrhizal root colonization (the role of fungal biofilters) with respect to molecular-level Pb perception by plant root cells. Biochemical, microscopic, proteomic and metabolomic studies were performed to determine the molecular status of Populus × canescens microcuttings grown in agar medium enriched with 0.75 mM Pb(NO<sub>3</sub>)<sub>2</sub>. Noninoculated and inoculated with Paxillus involutus poplars were analyzed in two independent comparisons of the corresponding control and Pb treatments. After six weeks of growth, Pb caused no negative phenotypic effects. No Pb-exposed poplar showed impaired growth or decreased leaf pigmentation. Proteomic signals of intensified Pb sequestration in the plant cell wall and vacuoles, cytoskeleton modifications, H<sup>+</sup>-ATPase-14-3-3 interactions, and stabilization of protein turnover in chronically Pb-exposed plants co-occurred with high metabolomic stability. There were no differentially abundant root primary metabolites; only a few differentially abundant root secondary metabolites and no Pb-triggered ROS burst were observed. Our results strongly suggest that proteome adjustments targeting Pb sequestration and ROS scavenging, which are considerably similar but less intensive in ectomycorrhizal poplars than in control poplars due to the P. involutus biofilter (as confirmed in a mineral study), were responsible for the metabolomic and phenotypic stability of poplars exposed to chronic mild Pb stress.</AbstractText><CopyrightInformation>Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Szuba</LastName><ForeName>Agnieszka</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035, Kórnik, Poland. Electronic address: aszuba@man.poznan.pl.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Marczak</LastName><ForeName>Łukasz</ForeName><Initials>Ł</Initials><AffiliationInfo><Affiliation>Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznań, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kozłowski</LastName><ForeName>Rafał</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Institute of Geography and Environmental Sciences, Jan Kochanowski University, Universytecka 7, 24-406, Kielce, Poland.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>04</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Environ Pollut</MedlineTA><NlmUniqueID>8804476</NlmUniqueID><ISSNLinking>0269-7491</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020543">Proteome</NameOfSubstance></Chemical><Chemical><RegistryNumber>2P299V784P</RegistryNumber><NameOfSubstance UI="D007854">Lead</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D007854" MajorTopicYN="N">Lead</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="Y">Mycorrhizae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="Y">Populus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020543" MajorTopicYN="N">Proteome</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Ectomycorrhiza</Keyword><Keyword MajorTopicYN="N">Heavy metals</Keyword><Keyword MajorTopicYN="N">Increased tolerance</Keyword><Keyword MajorTopicYN="N">Root metabolome</Keyword><Keyword MajorTopicYN="N">Root proteome</Keyword></KeywordList><CoiStatement>Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>12</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>03</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>04</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>5</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>7</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>5</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32387672</ArticleId><ArticleId IdType="pii">S0269-7491(19)37166-0</ArticleId><ArticleId IdType="doi">10.1016/j.envpol.2020.114585</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Pologne</li></country></list><tree><country name="Pologne"><noRegion><name sortKey="Szuba, Agnieszka" sort="Szuba, Agnieszka" uniqKey="Szuba A" first="Agnieszka" last="Szuba">Agnieszka Szuba</name></noRegion><name sortKey="Kozlowski, Rafal" sort="Kozlowski, Rafal" uniqKey="Kozlowski R" first="Rafał" last="Kozłowski">Rafał Kozłowski</name><name sortKey="Marczak, Lukasz" sort="Marczak, Lukasz" uniqKey="Marczak L" first="Łukasz" last="Marczak">Łukasz Marczak</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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